Investigating the Presence of Efflux Pump Genes adeI and adeJ in Clinical Antibiotic-Resistant Isolates of Acinetobacter baumannii

Background: Acinetobacter baumannii is one of the most important known causes hospital infections worldwide that resistant to many common antibiotics. Efflux pumps are among main reasons behind resistance in this bacterium. Objectives: This study was conducted investigate presence efflux pump genes (adeI, adeJ) clinical antibiotic-resistant isolates A. Tehran hospitals. Methods: One hundred fifty samples wounds, urine, sputum, and blood were collected periodically (6 months) from identified using biochemical methods. After conducting tests, final confirmation performed by examining blaOXA-51-like gene molecular polymerase chain reaction (PCR) method. The disk diffusion antimicrobial susceptibility testing Mueller Hinton agar growth medium according Clinical Laboratory Standards Institute (CLSI) guidelines on nine Then investigated for adeI adeJ genes. Results: Examining antibiotic showed level varied 48.1% 98.2%, depending type. In study, highest lowest tetracycline gentamicin, respectively. Also, positive tetracycline, amikamycin, ceftazidime, ceftriaxone. Isolates negative these two sensitivity imipenem, ciprofloxacin. Conclusions: correlation test PCR results significantly increased all Therefore, evaluating proves be useful identifying strains appropriate drug treatment. Of course, role other factors creating should not neglected.